Amino acids analysis Protocols by Catherine Cooper

By Catherine Cooper

A set of vintage and state-of-the-art options of excessive application in answering particular organic questions on amino acids. universal tools comprise these in line with HPLC or gasoline chromatography separation and research after precolumn derivatization. New ideas in line with capillary electrophoresis separation, high-performance anion alternate chromatography, and mass spectrometry also are awarded. each one technique is defined in step by step element to make sure profitable experimental effects and emphasizes pattern guidance, quite the gathering and garage of physically fluids. updated and hugely sensible, Amino Acid research Protocols bargains analytical and scientific chemists, in addition to a huge diversity of organic and biomedical investigators, a wealthy compendium of laboratory instruments for the efficient research of either universal and unusual amino acids.

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Extra resources for Amino acids analysis Protocols

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Equilibrate the column and detector with 90% solvent A and 10% solvent B. 3. Bring samples for analysis to room temperature; dilute, if necessary, with solvent A, and inject 20 µL (50–1000 pmol) onto the column (see Note 7). 4. Elute with gradient as described in Table 1 (see Note 8). Resolution of 2,4dinitrophenyl-5-L-alanine amide derivatives of 18 commonly occurring L-amino acids and of cysteic acid is achieved within 120 min (Fig. 2) (see Note 9). 5. Determine the response factor for each amino acid from the average peak area of standard amino acid chromatograms at 100, 250, 500, and 1000 pmol amounts.

Step 4) to the Dionex carbohydrate standard to obtain a 100-µM stock solution of each of the six components. Vortex to support the dissolution of the dry residue. 0 µM concentration of all four sugars and two amino sugars. 3. 1. Oxidation of Methionine and Cystine/Cysteine Prior to Hydrolysis 1. Prepare the performic acid reagent freshly from formic acid and hydrogen peroxide before each application. Mix 900 µL of the formic acid and 100 µL 30% hydrogen peroxide in a microcentrifuge tube. Vortex the mixture.

The small particle size of the anion–exchange layer exhibits adequate mass transfer characteristics enabling high chromatographic peak efficiency. The highly crosslinked core of the AminoPac PA10 packing makes it possible to use organic solvents to facilitate column cleanup. Use chemicals exactly as specified by catalog number and supplier. Consult with Dionex should one or more of the specified reagents be not available in your geographical area. Minimize any extraneous contamination of eluents.

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